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    目录产品 » 抗体产品 » 标签抗体 » TF-tag Antibody, pAb, Rabbit

    TF-tag Antibody, pAb, Rabbit

    Synonyms Rabbit Anti-TF-tag pAb;
    Description Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems.
    Trigger Factor (TF) is a prokaryotic ribosome-associated chaperone protein (48 kDa) which facilitates co-translational folding of newly expressed proteins, thus enabling efficient soluble protein production for otherwise intractable target proteins.

    GenScript Rabbit Anti-TF-tag polyclonal Antibody is developed in rabbit using purified recombinant TF protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
    Host Species Rabbit
    Conjugation Unconjugated
    Immunogen Purified recombinant TF protein
    Purification Immunoaffinity chromatography

    Subclass Rabbit IgG
    Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
    Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
    Specificity GenScript Rabbit Anti-TF-tag polyclonal antibody specifically reacts with fusion proteins containing TF tag. The antibody can also detect endogenous levels of E.coli trigger factor.
    Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.05-0.2 μg/ml
    Western blot: 0.2-1 μg/ml
    Immunoprecipitation (IP): 2-10 μg/mg of lysate
    Other Applications: user-optimized


    Eunyong Park and Tom A. Rapoport. Bacterial Protein Translocation Requires Only One Copy Of The Secy Complex In Vivo. J Cell Biol. 2012 Sep;198(5):881 - 893.

    Lin KF., et al. Cotranslational protein folding within the ribosome tunnel influences trigger-factor recruitment. Biophys J. 2012 Jun;102(12):2818-27.

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    产品编号: A01329
    规格
    价格
    2674 A01329-40 40 ug ¥990.00
    河南481开奖直播
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