|Synonyms||Rabbit Anti-DDDDK-tag pAb; Anti-DDDDK-tag pAb|
|Description||Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.
The xxxDDDDK-tag is a small size and high hydrophilicity epitope tag containing an enterokinase cleavage site that allows it to be completely removed from the fusion proteins.
The GenScript Rabbit Anti-DDDDK-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide containing xxxDDDDK epitope conjugated to KLH.
This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
|Immunogen||A synthetic epitope peptide xxxDDDDK conjugated to KLH. This sequence represents the enterokinase cleavage site (ECS).|
|Concentration||1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide|
|Reconstitution||Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.|
|Specificity||This Antibody recognizes the C-terminal enterokinase cleavage site and specifically binds to C-terminal xxxDDDDK tagged fusion proteins, such as Xpress tag or Flag tag.|
|Storage||The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 μg/ml
Western blot: 0.1-1.0 μg/ml
Western Blot Using the ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) (GenScript, L00204C) is recommended. 5 μg of this antibody is mixed with 10 ml of WB solution for any 8 cm X 8 cm membrane.
Other applications: user-optimized